TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= TBB_ENCCU Q8SS99 Tubulin beta chain (Beta-tubulin) Length=439 Score E Sequences producing significant alignments: (Bits) Value unplaced-665 724 0.0 scaffold-26 674 0.0 scaffold-57 346 2e-106 unplaced-5 345 2e-106 scaffold-423 152 3e-47 > unplaced-665 Length=20473 Score = 724 bits (1870), Expect = 0.0, Method: Compositional matrix adjust. Identities = 355/456 (78%), Positives = 395/456 (87%), Gaps = 22/456 (5%) Frame = -2 Query 1 MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTGRYVGTSDNQLERIN------------ 48 MREI+HL TG CGN +G KFWE IS EHGID GRY G SD QLERIN Sbjct 7236 MREIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYEGDSDLQLERINGEFLNVLFCA** 7057 Query 49 ----------VYYNEASSKKYVPRAVLIDLEPGTMDAVRQGPFGELFRPDNFVFGQSGAG 98 VY+NEAS KYVPRAVL+DLEPGTMD+VR GP+G LFRPDNF+FGQSGAG Sbjct 7056 AFCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAG 6877 Query 99 NNWAKGHYTEGAELIDSVMDVVRKEAESSDCLQGFQITHSlgggtgagmgtlllSKIRED 158 NNWAKGHYTEGAEL+DSV+DVVRKEAE DCLQGFQITHSLGGGTGAGMGTLL+SKIRE+ Sbjct 6876 NNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREE 6697 Query 159 FPDRMICTFSVVPSPKVSDTVVEPYNATLSIHQLVENADETFCIDNEALYDICFRTLKLN 218 +PDRM+CTFSVVPSPKVSDTVVEPYNATLS+HQLVEN+DETFCIDNEALYDICFRTLKL Sbjct 6696 YPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLT 6517 Query 219 NPGYGDLNHLVSLVMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFVAGFAPLIAIGT 278 P YGDLNHLVS VMSGVTT +RFPGQLNADLRKLAVNM+PFPRLHFF+ GFAPL + G+ Sbjct 6516 TPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGS 6337 Query 279 QKFKTYSVSELTQQMFDSKNMMTACDPRKGRYLTVAAMFRGKISMKDVDEQMSMVQSKNS 338 Q+++ SV+ELT QMFD+KNMM A DPR GRYL VAA+FRGK+SMK+VDEQM VQ+KNS Sbjct 6336 QQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNS 6157 Query 339 SLFVEWIPSNVKTAVCDIAPTGLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWY 398 S FVEWIP+NVKTAVCDI P GL+MSATF+GN+T+IQELFKRISDQF+VMF+RKAFLHWY Sbjct 6156 SYFVEWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 5977 Query 399 TGEGMDEMEFSEAESNMNDLLSEYQQYQDATIEDAE 434 TGEGMDEMEF+EAESNMNDL+SEYQQYQDAT E+ + Sbjct 5976 TGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEED 5869 > scaffold-26 Length=483268 Score = 674 bits (1738), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/392 (83%), Positives = 361/392 (92%), Gaps = 0/392 (0%) Frame = -3 Query 43 QLERINVYYNEASSKKYVPRAVLIDLEPGTMDAVRQGPFGELFRPDNFVFGQSGAGNNWA 102 QL I VYYNEAS KYVPRAVL+DLEPGTMD+VR GP+G LFRPDNF+FGQSGAGNNWA Sbjct 109811 QLYLIVVYYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAGNNWA 109632 Query 103 KGHYTEGAELIDSVMDVVRKEAESSDCLQGFQITHSlgggtgagmgtlllSKIREDFPDR 162 KGHYTEGAEL+DSV+DVVRKEAE DCLQGFQITHSLGGGTGAGMGTLL+SKIRE++PDR Sbjct 109631 KGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDR 109452 Query 163 MICTFSVVPSPKVSDTVVEPYNATLSIHQLVENADETFCIDNEALYDICFRTLKLNNPGY 222 M+CTFSVVPSPKVSDTVVEPYNATLS+HQLVEN+DETFCIDNEALYDICFRTLKL P Y Sbjct 109451 MMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTY 109272 Query 223 GDLNHLVSLVMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFVAGFAPLIAIGTQKFK 282 GDLNHLVS VMSGVTT +RFPGQLNADLRKLAVNM+PFPRLHFF+ GFAPL + G+Q+++ Sbjct 109271 GDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGSQQYR 109092 Query 283 TYSVSELTQQMFDSKNMMTACDPRKGRYLTVAAMFRGKISMKDVDEQMSMVQSKNSSLFV 342 SV+ELT QMFD+KNMM A DPR GRYL VAA+FRGK+SMK+VDEQM VQ+KNSS FV Sbjct 109091 ALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFV 108912 Query 343 EWIPSNVKTAVCDIAPTGLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGEG 402 EWIP+NVKTAVCDI P GL+MSATF+GN+T+IQELFKRISDQF+VMF+RKAFLHWYTGEG Sbjct 108911 EWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWYTGEG 108732 Query 403 MDEMEFSEAESNMNDLLSEYQQYQDATIEDAE 434 MDEMEF+EAESNMNDL+SEYQQYQDAT E+ + Sbjct 108731 MDEMEFTEAESNMNDLVSEYQQYQDATAEEED 108636 Score = 73.2 bits (178), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 35/48 (73%), Positives = 37/48 (77%), Gaps = 0/48 (0%) Frame = -2 Query 1 MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTGRYVGTSDNQLERIN 48 MREI+HL TG CGN +G KFWE IS EHGID GRY G SD QLERIN Sbjct 110007 MREIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYEGDSDLQLERIN 109864 > scaffold-57 Length=110052 Score = 346 bits (887), Expect = 2e-106, Method: Compositional matrix adjust. Identities = 177/437 (41%), Positives = 266/437 (61%), Gaps = 16/437 (4%) Frame = -2 Query 1 MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTG-----RYVGTSDNQLERINVYYNEAS 55 MRE I + GQ G Q+G WE EHGI G + VG D+ N +++E Sbjct 88586 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSF---NTFFSETG 88416 Query 56 SKKYVPRAVLIDLEPGTMDAVRQGPFGELFRPDNFVFGQSGAGNNWAKGHYTEGAELIDS 115 + K+VPR V DLEP +D VR G + +LF P+ + G+ A NN+A+GHYT G E+IDS Sbjct 88415 AGKHVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDS 88236 Query 116 VMDVVRKEAESSDCLQGFQITHSlgggtgagmgtlllSKIREDFPDRMICTFSVVPSPKV 175 V++ VRK A++ L GF + HS GGGTG+G G LLL ++ D+ + F+V P+P+V Sbjct 88235 VLERVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQV 88056 Query 176 SDTVVEPYNATLSIHQLVENADETFCIDNEALYDICFRTLKLNNPGYGDLNHLVSLVMSG 235 S VVEPYN+ L+ H +E++D F +DNEA+YDIC R L + P Y +LN L++ V+S Sbjct 88055 STAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSS 87876 Query 236 VTTCLRFPGQLNADLRKLAVNMIPFPRLHFFVAGFAPLIAIGTQKFKTYSVSELTQQMFD 295 +T LRF G LN DL + N++P+PR+HF +A +AP+I+ + SV E+TQ F+ Sbjct 87875 ITASLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFE 87696 Query 296 SKNMMTACDPRKGRYLTVAAMFRGKISMKDVDEQMSMVQSKNSSLFVEWIPSNVKTAVCD 355 N M CDPR G+Y+ ++RG + KDV+ ++ +++K + FV+W P+ K + Sbjct 87695 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 87516 Query 356 IAPT--------GLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGEGMDEME 407 PT + + + NTT+I E + R+ +F +M+ ++AF+HWY GEGM+E E Sbjct 87515 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGE 87336 Query 408 FSEAESNMNDLLSEYQQ 424 FSEA ++ L +Y++ Sbjct 87335 FSEAREDLAALEKDYEE 87285 > unplaced-5 Length=12763 Score = 345 bits (886), Expect = 2e-106, Method: Compositional matrix adjust. Identities = 176/437 (40%), Positives = 266/437 (61%), Gaps = 16/437 (4%) Frame = +2 Query 1 MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTG-----RYVGTSDNQLERINVYYNEAS 55 MRE I + GQ G Q+G WE EHGI G + VG D+ N +++E Sbjct 3257 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSF---NTFFSETG 3427 Query 56 SKKYVPRAVLIDLEPGTMDAVRQGPFGELFRPDNFVFGQSGAGNNWAKGHYTEGAELIDS 115 + K+VPR V DLEP +D VR G + +LF P+ + G+ A NN+A+GHYT G E+IDS Sbjct 3428 AGKHVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDS 3607 Query 116 VMDVVRKEAESSDCLQGFQITHSlgggtgagmgtlllSKIREDFPDRMICTFSVVPSPKV 175 V++ VRK A++ L GF + HS GGGTG+G G LLL ++ D+ + F+V P+P++ Sbjct 3608 VLERVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQI 3787 Query 176 SDTVVEPYNATLSIHQLVENADETFCIDNEALYDICFRTLKLNNPGYGDLNHLVSLVMSG 235 S VVEPYN+ L+ H +E++D F +DNEA+YDIC R L + P Y +LN L++ V+S Sbjct 3788 STAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSS 3967 Query 236 VTTCLRFPGQLNADLRKLAVNMIPFPRLHFFVAGFAPLIAIGTQKFKTYSVSELTQQMFD 295 +T LRF G LN DL + N++P+PR+HF +A +AP+I+ + SV E+TQ F+ Sbjct 3968 ITASLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFE 4147 Query 296 SKNMMTACDPRKGRYLTVAAMFRGKISMKDVDEQMSMVQSKNSSLFVEWIPSNVKTAVCD 355 N M CDPR G+Y+ ++RG + KDV+ ++ +++K + FV+W P+ K + Sbjct 4148 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 4327 Query 356 IAPT--------GLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGEGMDEME 407 PT + + + NTT+I E + R+ +F +M+ ++AF+HWY GEGM+E E Sbjct 4328 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGE 4507 Query 408 FSEAESNMNDLLSEYQQ 424 FSEA ++ L +Y++ Sbjct 4508 FSEAREDLAALEKDYEE 4558 > scaffold-423 Length=1446679 Score = 152 bits (385), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 112/397 (28%), Positives = 195/397 (49%), Gaps = 50/397 (13%) Frame = -2 Query 80 PFGELFRPDNFVFG--QSGAGNNWAKGHYTEGAELIDSVMDVVRKEAESSDCLQGFQITH 137 P+G L P+N SGAGNNWA G Y G L + + D++ +EA++++ L+GF + H Sbjct 1101339 PYGLLHNPENVY*S*DGSGAGNNWASG-YQSGGRLSEDLFDILDREADNAESLEGFNLMH 1101163 Query 138 SlgggtgagmgtlllSKIREDFPDRMICTFSVVPS--PKVSDTVVEPYNATLSIHQLVEN 195 S+ GGTG+G+G+ LL + + FP ++I T+SV PS VSD VV+PYN+ LS+ +L EN Sbjct 1101162 SIAGGTGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSVLSLKRLTEN 1100983 Query 196 ADETFCIDNEALYDICFRTLKLNNPGYGDLNHLVSLVMSGVTTCLRFPGQLNADLRKLAV 255 AD +DN AL + L + N + +N +VS +M+ + +RFP + L Sbjct 1100982 ADMVTVLDNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMS 1100803 Query 256 NMIPFPRLHFFVAGFAPLIAIGTQKF--------------------------KTYSVSEL 289 +++P + H+ ++P + + K +S++ Sbjct 1100802 SLVPIRKCHYITCAYSPFTHNDLRDYAASSNVNAAASENSANAVNRSR*SLMKKAGLSDI 1100623 Query 290 TQQMFDSKNMMTACDPRKGR-YLTVAAMFRGKISMKDVDEQMSMVQSKNSSLFVEWIPSN 348 +++ +K+ + + + +L+ + G + KDV + + ++ KN+ F W PS+ Sbjct 1100622 MRRLL*TKSRVISVENTPLNCFLSAMTLV*GDVGAKDVHKAILKLKEKNTMQFAPWKPSS 1100443 Query 349 V-----KTAVC--DIAPTGLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGE 401 + + + C D P +S + N TS+ +LFK DQ+ + +R AFL Y Sbjct 1100442 M*INVARRSPCLEDGDPQSGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKL 1100263 Query 402 GMDEMEFSEAESNMNDLLSEYQQYQDATIEDAEEFLV 438 M D L E+ +++ +E EE+L Sbjct 1100262 DM-----------FKDGLEEFDVSRESLLEVTEEYLA 1100185 Score = 47.4 bits (111), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 20/63 (32%), Positives = 36/63 (57%), Gaps = 8/63 (13%) Frame = -1 Query 16 VGCKFWETISGEHGIDQTGRYVGTSD--------NQLERINVYYNEASSKKYVPRAVLID 67 VG +FW + EHG+ G + +SD ++R ++++ +A Y+PR++L+D Sbjct 1101622 VGMEFWAQLCAEHGLAPDGLPLTSSD****SSSPPLMDRKDIFFYQAEDGHYIPRSILVD 1101443 Query 68 LEP 70 LEP Sbjct 1101442 LEP 1101434 Score = 27.7 bits (60), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 11/13 (85%), Positives = 11/13 (85%), Gaps = 0/13 (0%) Frame = -3 Query 2 REIIHLQTGQCGN 14 REII LQ GQCGN Sbjct 1101728 REIISLQVGQCGN 1101690 Score = 154 bits (388), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 114/396 (29%), Positives = 195/396 (49%), Gaps = 49/396 (12%) Frame = +3 Query 80 PFGELFRPDNFVFG--QSGAGNNWAKGHYTEGAELIDSVMDVVRKEAESSDCLQGFQITH 137 P+G L P+N SGAGNNWA G Y G L + + D++ +EA++S+ L+GF + H Sbjct 190311 PYGLLHNPENVY*S*DGSGAGNNWASG-Y*SGERLSEDLFDILDREADNSESLEGFNLMH 190487 Query 138 SlgggtgagmgtlllSKIREDFPDRMICTFSVVPS--PKVSDTVVEPYNATLSIHQLVEN 195 S+ GGTG+G+G+ LL + + FP ++I T+SV PS VSD VV+PYN+ LS+ +L EN Sbjct 190488 SIAGGTGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSLLSLKRLTEN 190667 Query 196 ADETFCIDNEALYDICFRTLKLNNPGYGDLNHLVSLVMSGVTTCLRFPGQLNADLRKLAV 255 AD +DN AL + L + N + +N +VS +M+ + +RFP + L Sbjct 190668 ADMVTVLDNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMS 190847 Query 256 NMIPFPRLHFFVAGFAPLI----------------------AIGTQK---FKTYSVSELT 290 +++P + H+ + P A+ + K +S++ Sbjct 190848 SLVPIRKCHYITCAYTPFTHNDLRDYAAISNVNAASENSTNAVNRSR*SLMKKAGLSDIM 191027 Query 291 QQMFDSKNMMTACDPRKGR-YLTVAAMFRGKISMKDVDEQMSMVQSKNSSLFVEWIPSNV 349 +++ K+ + + + YL+ ++ +G + KDV + + ++ KN+ F W PS++ Sbjct 191028 RRLL*PKSRVISVENTPLNCYLSAMSIVQGDVGAKDVHKAILKLKEKNTMQFAPWKPSSM 191207 Query 350 KTAVCDIAPT-------GLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGEG 402 V +P G +S + N TS+ +LFK DQ+ + +R AFL Y Sbjct 191208 *INVARRSPCLEDGDT*GGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLD 191387 Query 403 MDEMEFSEAESNMNDLLSEYQQYQDATIEDAEEFLV 438 M D L E+ +++ ++ EE+L Sbjct 191388 M-----------FKDGLEEFDVSRESLLDVTEEYLA 191462 Score = 47.0 bits (110), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 23/80 (29%), Positives = 39/80 (49%), Gaps = 18/80 (23%) Frame = +2 Query 9 TGQCGNQVGCKFWETISGEHGIDQTGRYVGTSD------------------NQLERINVY 50 TG + VG +FW + EHG+ G + +SD ++R +++ Sbjct 189959 TGMLLSVVGMEFWAQLCAEHGLAPDGLPLTSSD**************SPSIPLMDRKDIF 190138 Query 51 YNEASSKKYVPRAVLIDLEP 70 + +A Y+PR++LIDLEP Sbjct 190139 FYQAEDGHYIPRSILIDLEP 190198 Score = 27.3 bits (59), Expect(3) = 3e-47, Method: Compositional matrix adjust. Identities = 11/13 (85%), Positives = 11/13 (85%), Gaps = 0/13 (0%) Frame = +3 Query 2 REIIHLQTGQCGN 14 REII LQ GQCGN Sbjct 189873 REIISLQVGQCGN 189911 Lambda K H a alpha 0.320 0.134 0.399 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2635964994 Database: X5.fasta Posted date: Dec 25, 2021 2:56 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40