Buev Vitaly
+7 (925) 201-51-31
vittaria.fern@gmail.com
DoB: 25/09/2001 (20 y.o.)
Moscow, Russia
EXPERIENCE
Belozersky Institute Of Physico-Chemical Biology,
Department of structure and function of RNA
Supervisor - Prof. Dr. Petr V. Sergiev
AUG 2022 - PRESENT
Projects:
●High-throughput screening of new autophagy inducers
●The eect of putative methyltransferase NSUN7 on the late stages of mouse
spermiogenesis
Supervisor - Dr. Ilya A. Osterman
FEBR 2020 - AUG 2022
Projects:
●Search for potential translational inhibitors in chemical libraries
●Studying of mechanism of action of antibiotic Althiomycin
EDUCATION
Lomonosov Moscow State University, Faculty of Bioengineering and
Bioinformatics
Expected degree: Specialist degree in “Bioengineering and Bioinformatics”
(equivalent to an MS)
GPA: 4,52
2019 - expected graduation 2025
PUBLICATIONS
●D. A. Lukianov, V. S. Buev, Y. A. Ivanenkov, V. G. Kartsev, D. A. Skvortsov, I. A. Osterman,
and P. V. Sergiev. Imidazole derivative as a novel translation inhibitor. Acta Naturae
(англоязычная версия), 14(2):71–77, 2022.
https://doi.org/10.32607/actanaturae.11654
●Z. Z. Khairullina, G. I. Makarov, A. G. Tereshchenkov, V. S. Buev, D. A. Lukianov, V. I.
Polshakov, V. N. Tashlitsky, I. A. Osterman, and N. V. Sumbatyan. Conjugates of
desmycosin with fragments of antimicrobial peptide oncocin: Synthesis, antibacterial
activity, interaction with ribosome. Biochemistry (Moscow), 87(9):871–889, 2022
https://doi.org/10.1134/S0006297922090024
CONFERENCE PARTICIPATION
●International Conference Of Students, Postgraduates And Young Scientists “Lomonosov
- 2021”, “Search for potential translation inhibitors in Chemical libraries” (Poster)
Moscow, Russia, April 12-23, 2021
●International Conference Of Students, Postgraduates And Young Scientists “Lomonosov
- 2022” “Study of the mechanism of action of the antibiotic Altiomycin” (Poster)
Moscow, Russia, April 11-22, 2022
INTERNSHIPS
Peter the Great Saint Petersburg Polytechnic University,
Laboratory for Molecular Neurodegeneration
Supervisor - Dr. Bezprozvanny I.B.
AUG 2022
Project:
●Analysis of STIM1/STIM2 impact on Store-Operated Calcium Entry (SOCE)
RESEARCH SKILLS
Genetic engineering
Extraction of plasmid or genome DNA; Transformation; Restriction;
Ligation; In vitro translation inhibition; Transfection (with PEI)
DNA and RNA
Gel electrophoresis; DNA and RNA extraction; PCR; RT-qPCR; Sanger
sequencing; Toeprint (primer extension inhibition)
Protein
SDS-PAGE; Ion-exchange and Gel-filtration chromatography; HPLC;
Bradford assay; Western blotting
Bacteriology
Maintenance of bacterial culture; Determination of Minimum
Inhibitory Concentration (MIC); Selection of resistance clones
Cell and tissue
Maintenance of cell culture (SH-SY5; HEK-293T; HeLa); testis cells
separation
Microscopy
Confocal microscopy; IHC; ICC
Bioinformatics
Python; R; JailView; MEME; MEGA; PyMol; analyzing of NGS and
Nanopore sequencing
Other
Calcium Imaging; Submitochondrial particles (Keilin and Hartree
preparation)
LANGUAGES
English (advanced), Russian (native), French (beginner)
