As it can be seen from Table 1, searching with the standart parameters resulted in 100 finds where all had E-value 0.0. However, after changing parameters the amount of homologous with E-value lower than 0.001 decreased (not all had such low E parameter)
Table 1. Results of BLAST search
| Amount of results | E-value < 0,001 | max E-value | Limited by |
| 100 | 100 | 0 | max amount of results |
| 500 | 497 | 5,1 | max amount of results |
From Table 2 it can be seen that after changing word-length from 6 to 2 the amount of finds with E-value lower than 0.001 decreased while the max E-value increased.
Table 2. Results of word-length changing
| word-length | Amount of results | E-value < 0,001 | max E-value | |
| 6 | 500 | 497 | 5,1 | |
| 2 | 500 | 480 | 9,1 | |
After changing bank size, having limited it by 'Proteobacteria' organism, E-value decreased which confermed the hypothesis of reduction E-value with decrease of bank size.
Table 3. Results of bank size changing
| ID | AC | Organism | Taxonomy | E-value | Organism search | Amount of results | E-value after changing parameters |
| KATG_RHOCA | P37743 | Rhodobacter capsulatus | Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Rhodobacter | 4,00E-158 | Proteobacteria | 250 | 1,00E-158 |