Sanger sequencing

Task 1. Сhromatograms

Unreadable parts were found and deleted: in the direct read 1-19 and 677-717, and in the reverse read 1-53 and 688-699. Table 1 explains some problems were solved during correcting of reads.

PictureDescription

The signal of Thymine was overlayed with the sygnal of Adenine, but reverse read brings unambiguity

This situation could be explained as polymorphism, but direct read brings unambiguity

The same situation. The problem, however, was with direct read.
Table 1. Chromatogram problems

Task 2. Unreadable part of chromatogram

For this task L46_COI_F_A07_WSBS-Seq-07-10-16.ab1 was used. It's impossible to read this chromatogram due to blurred peaks, high noise level and pollution of the sample. Part of file is presented in Fig. 1.

Figure 1. Unreadable part of L46_COI_F_A07_WSBS-Seq-07-10-16.ab1.

Contacts: vorobiovarita@kodomo.fbb.msu.ru

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