TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= CYB_SPIPN Q950S3 Cytochrome b (Complex III subunit 3) (Complex III subunit III) (Cytochrome b-c1 complex subunit 3) (Ubiquinol-cytochrome-c reductase complex cytochrome b subunit) Length=396 Score E Sequences producing significant alignments: (Bits) Value unplaced-887 207 6e-58 unplaced-63 136 2e-37 scaffold-451 28.9 6.3 > unplaced-887 Length=30515 Score = 207 bits (527), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 127/220 (58%), Positives = 155/220 (70%), Gaps = 0/220 (0%) Frame = -1 Query 144 ATVITNLLSAIPWIGTDFVLFVWGGFSVDNATLNRFFSLHYLLPFIlaalvvmhllalhQ 203 ATVITNLLSAIP++G D V+ VWGGFSV N TLNRF+SLHYLLPFIL LV HL ALH+ Sbjct 7403 ATVITNLLSAIPYLGQDLVILVWGGFSVGNPTLNRFYSLHYLLPFILLGLVAYHLSALHE 7224 Query 204 DGSNNPEGISSSSDRLRFHPYFTSKDLVGFVWMAILLSIFVFFAPYYLGHPDNSIPANPL 263 SNNP GISS++D++ FHPYFT KDL F IL S VF+ P YL HPDN IPANPL Sbjct 7223 HASNNPLGISSNTDKIYFHPYFTIKDLYYFTLFLILFSTLVFYFPNYLNHPDNYIPANPL 7044 Query 264 VTPHSIVPEWYFLPFYAILRAIPSKLGGVIAMFGALLILFPLALIHTNNVRSNRYRPLLN 323 VTP SI PEWY L YAILR IP+KL GV+A+ +LLIL L I +RS R Sbjct 7043 VTPLSIQPEWYLLAPYAILRTIPNKLLGVVALALSLLILLILPFISQFAIRSYTIRSYSK 6864 Query 324 LLFWIFVFNFFVLLWVGAKPIAQPYILIGQISTIIYFLYF 363 + +W+F+ ++ +L+ GA PI++ I + +S+I YF YF Sbjct 6863 IFYWLFIGSYLLLVICGALPISEEIINLSILSSIYYFSYF 6744 Score = 149 bits (375), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 73/119 (61%), Positives = 92/119 (77%), Gaps = 0/119 (0%) Frame = -2 Query 9 ILVLVNDFVIDSPLPTNLTYFWNFGSLLGVNLVILIISGLTLAMHYTPNTLLAFSSVEHI 68 ++ L D+ + S LP+N++ WN+GSLLGV LVI I++G+TL MHY N L+F+ VEHI Sbjct 9889 MIKLFTDYFVYSGLPSNISINWNYGSLLGVILVIQIMTGVTLGMHYAANIELSFNLVEHI 9710 Query 69 MRDVNNGWLLRYIHANGASFFFIWVYLHIGRNLYYGSYRSPRGLLWSIGVVIFILMMAT 127 MRD+N GWL+RYIHANGAS FF++VYLHI R LY GSY PR LW+IGVVI+ILMM Sbjct 9709 MRDINAGWLVRYIHANGASLFFLFVYLHIARGLYAGSYTYPRHKLWNIGVVIYILMMGE 9533 Score = 35.8 bits (81), Expect = 0.041, Method: Compositional matrix adjust. Identities = 14/17 (82%), Positives = 16/17 (94%), Gaps = 0/17 (0%) Frame = -1 Query 127 TAFIGYVLPWGQMSFWG 143 TAF+GYVLP+GQMS WG Sbjct 8585 TAFLGYVLPFGQMSIWG 8535 > unplaced-63 Length=685 Score = 136 bits (343), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 71/166 (43%), Positives = 102/166 (61%), Gaps = 0/166 (0%) Frame = +1 Query 203 QDGSNNPEGISSSSDRLRFHPYFTSKDLVGFVWMAILLSIFVFFAPYYLGHPDNSIPANP 262 Q GS NP GI++ SD + F+PYF +KDLV + ++ + V F P LGHPDN+IPANP Sbjct 25 QYGSTNPLGINAQSDLVDFYPYFYTKDLVAALRLSFGAAYLVNFNPELLGHPDNNIPANP 204 Query 263 LVTPHSIVPEWYFLPFYAILRAIPSKLGGVIAMFGALLILFPLALIHTNNVRSNRYRPLL 322 TP IVPEWYFL YAILR+IP+KL GV A+ L L +H + R +R L Sbjct 205 YATPRHIVPEWYFLWVYAILRSIPNKLAGVRAIGLVFAALAALPYMHKPSCRGPSFRRLH 384 Query 323 NLLFWIFVFNFFVLLWVGAKPIAQPYILIGQISTIIYFLYFVILMI 368 + W + +F +L ++G +P+ PY+++GQ T+ +F + L + Sbjct 385 KV*VWALIGDFMLLSYLGQQPVEPPYVVLGQRRTVRFFSLLIGLCV 522 > scaffold-451 Length=486760 Score = 28.9 bits (63), Expect = 6.3, Method: Compositional matrix adjust. Identities = 11/25 (44%), Positives = 16/25 (64%), Gaps = 0/25 (0%) Frame = +2 Query 351 IGQISTIIYFLYFVILMILGCIMTY 375 +G + +IY LYF L+IL C+ Y Sbjct 439802 VGDAAKVIYHLYFEYLIILTCVDEY 439876 Lambda K H a alpha 0.333 0.148 0.492 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2308971976 Database: X5.fasta Posted date: Nov 1, 2020 9:58 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40