TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= TBB4A_HUMAN P04350 Tubulin beta-4A chain (Tubulin 5 beta) (Tubulin beta-4 chain) Length=444 Score E Sequences producing significant alignments: (Bits) Value unplaced-665 781 0.0 scaffold-26 710 0.0 unplaced-5 346 4e-105 scaffold-57 347 5e-105 scaffold-423 153 2e-46 scaffold-693 30.8 1.6 > unplaced-665 Length=20473 Score = 781 bits (2016), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/449 (86%), Positives = 411/449 (92%), Gaps = 22/449 (5%) Frame = -2 Query 1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERIN------------ 48 MREIVHLQ GQCGNQIGAKFWEVISDEHGIDP G Y GDSDLQLERIN Sbjct 7236 MREIVHLQTGQCGNQIGAKFWEVISDEHGIDPNGRYEGDSDLQLERINGEFLNVLFCAQ* 7057 Query 49 ----------VYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAG 98 VY+NEA+GG YVPRAVLVDLEPGTMDSVR+GP+G +FRPDNF+FGQSGAG Sbjct 7056 AFCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAG 6877 Query 99 NNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQlthslgggtgsgmgtllisKIREE 158 NNWAKGHYTEGAELVD+VLDVVRKEAE CDCLQGFQ+THSLGGGTG+GMGTLLISKIREE Sbjct 6876 NNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREE 6697 Query 159 FPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLT 218 +PDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVEN+DET+CIDNEALYDICFRTLKLT Sbjct 6696 YPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLT 6517 Query 219 TPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGS 278 TPTYGDLNHLVSA MSGVTT +RFPGQLNADLRKLAVNMVPFPRLHFFM GFAPLTSRGS Sbjct 6516 TPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGS 6337 Query 279 QQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNS 338 QQYRAL+V ELT QMFDAKNMMAA DPRHGRYL VAA+FRG+MSMKEVDEQMLSVQ+KNS Sbjct 6336 QQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNS 6157 Query 339 SYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWY 398 SYFVEWIPNNVKTAVCDIPP+GLKM+ATFIGNSTAIQELFKRIS+QF+ MF+RKAFLHWY Sbjct 6156 SYFVEWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 5977 Query 399 TGEGMDEMEFTEAESNMNDLVSEYQQYQD 427 TGEGMDEMEFTEAESNMNDLVSEYQQYQD Sbjct 5976 TGEGMDEMEFTEAESNMNDLVSEYQQYQD 5890 > scaffold-26 Length=483268 Score = 710 bits (1833), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/385 (90%), Positives = 370/385 (96%), Gaps = 0/385 (0%) Frame = -3 Query 43 QLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWA 102 QL I VYYNEA+GG YVPRAVLVDLEPGTMDSVR+GP+G +FRPDNF+FGQSGAGNNWA Sbjct 109811 QLYLIVVYYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAGNNWA 109632 Query 103 KGHYTEGAELVDAVLDVVRKEAESCDCLQGFQlthslgggtgsgmgtllisKIREEFPDR 162 KGHYTEGAELVD+VLDVVRKEAE CDCLQGFQ+THSLGGGTG+GMGTLLISKIREE+PDR Sbjct 109631 KGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDR 109452 Query 163 IMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTY 222 +M TFSVVPSPKVSDTVVEPYNATLSVHQLVEN+DET+CIDNEALYDICFRTLKLTTPTY Sbjct 109451 MMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTY 109272 Query 223 GDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR 282 GDLNHLVSA MSGVTT +RFPGQLNADLRKLAVNMVPFPRLHFFM GFAPLTSRGSQQYR Sbjct 109271 GDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGSQQYR 109092 Query 283 ALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFV 342 AL+V ELT QMFDAKNMMAA DPRHGRYL VAA+FRG+MSMKEVDEQMLSVQ+KNSSYFV Sbjct 109091 ALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFV 108912 Query 343 EWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEG 402 EWIPNNVKTAVCDIPP+GLKM+ATFIGNSTAIQELFKRIS+QF+ MF+RKAFLHWYTGEG Sbjct 108911 EWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWYTGEG 108732 Query 403 MDEMEFTEAESNMNDLVSEYQQYQD 427 MDEMEFTEAESNMNDLVSEYQQYQD Sbjct 108731 MDEMEFTEAESNMNDLVSEYQQYQD 108657 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 44/48 (92%), Positives = 44/48 (92%), Gaps = 0/48 (0%) Frame = -2 Query 1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERIN 48 MREIVHLQ GQCGNQIGAKFWEVISDEHGIDP G Y GDSDLQLERIN Sbjct 110007 MREIVHLQTGQCGNQIGAKFWEVISDEHGIDPNGRYEGDSDLQLERIN 109864 > unplaced-5 Length=12763 Score = 346 bits (888), Expect = 4e-105, Method: Compositional matrix adjust. Identities = 173/434 (40%), Positives = 268/434 (62%), Gaps = 10/434 (2%) Frame = +2 Query 1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGN 58 MRE + + GQ G QIG WE+ EHGI P GT D + + + N +++E G Sbjct 3257 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGK 3436 Query 59 YVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLD 118 +VPR V DLEP +D VR+G + Q+F P+ + G+ A NN+A+GHYT G E++D+VL+ Sbjct 3437 HVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDSVLE 3616 Query 119 VVRKEAESCDCLQGFQlthslgggtgsgmgtllisKIREEFPDRIMNTFSVVPSPKVSDT 178 VRK A++C LQGF + HS GGGTGSG G LL+ ++ ++ + F+V P+P++S Sbjct 3617 RVRKLADNCTGLQGFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQISTA 3796 Query 179 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTT 238 VVEPYN+ L+ H +E++D + +DNEA+YDIC R L + P+Y +LN L++ +S +T Sbjct 3797 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 3976 Query 239 CLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKN 298 LRF G LN DL + N+VP+PR+HF + +AP+ S + L+V E+TQ F+ N Sbjct 3977 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFEPNN 4156 Query 299 MMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPP 358 M CDPR+G+Y+ ++RG + K+V+ + ++++K + FV+W P K + PP Sbjct 4157 QMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 4336 Query 359 R--------GLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410 + A + N+TAI E + R+ +F M+ ++AF+HWY GEGM+E EF+E Sbjct 4337 TVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 4516 Query 411 AESNMNDLVSEYQQ 424 A ++ L +Y++ Sbjct 4517 AREDLAALEKDYEE 4558 > scaffold-57 Length=110052 Score = 347 bits (889), Expect = 5e-105, Method: Compositional matrix adjust. Identities = 174/434 (40%), Positives = 268/434 (62%), Gaps = 10/434 (2%) Frame = -2 Query 1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGN 58 MRE + + GQ G QIG WE+ EHGI P GT D + + + N +++E G Sbjct 88586 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGK 88407 Query 59 YVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLD 118 +VPR V DLEP +D VR+G + Q+F P+ + G+ A NN+A+GHYT G E++D+VL+ Sbjct 88406 HVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDSVLE 88227 Query 119 VVRKEAESCDCLQGFQlthslgggtgsgmgtllisKIREEFPDRIMNTFSVVPSPKVSDT 178 VRK A++C LQGF + HS GGGTGSG G LL+ ++ ++ + F+V P+P+VS Sbjct 88226 RVRKLADNCTGLQGFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQVSTA 88047 Query 179 VVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTT 238 VVEPYN+ L+ H +E++D + +DNEA+YDIC R L + P+Y +LN L++ +S +T Sbjct 88046 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 87867 Query 239 CLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKN 298 LRF G LN DL + N+VP+PR+HF + +AP+ S + L+V E+TQ F+ N Sbjct 87866 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFEPNN 87687 Query 299 MMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPP 358 M CDPR+G+Y+ ++RG + K+V+ + ++++K + FV+W P K + PP Sbjct 87686 QMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 87507 Query 359 R--------GLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTE 410 + A + N+TAI E + R+ +F M+ ++AF+HWY GEGM+E EF+E Sbjct 87506 TVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 87327 Query 411 AESNMNDLVSEYQQ 424 A ++ L +Y++ Sbjct 87326 AREDLAALEKDYEE 87285 > scaffold-423 Length=1446679 Score = 153 bits (387), Expect(2) = 2e-46, Method: Compositional matrix adjust. Identities = 109/391 (28%), Positives = 201/391 (51%), Gaps = 43/391 (11%) Frame = +3 Query 73 MDSVRSGPFGQIFRPDNFVFGQ--SGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCL 130 ++++ P+G + P+N Q SGAGNNWA G Y G L + + D++ +EA++ + L Sbjct 190290 INNIMQSPYGLLHNPENVYQSQDGSGAGNNWASG-YQSGERLSEDLFDILDREADNSESL 190466 Query 131 QGFQlthslgggtgsgmgtllisKIREEFPDRIMNTFSVVPS--PKVSDTVVEPYNATLS 188 +GF L HS+ GGTGSG+G+ L+ + + FP +++ T+SV PS VSD VV+PYN+ LS Sbjct 190467 EGFNLMHSIAGGTGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSLLS 190646 Query 189 VHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNA 248 + +L EN D +DN AL + L + ++ +N +VS M+ + +RFP Sbjct 190647 LKRLTENADMVTVLDNAALNRLAQDQLHIKNASFAQINRMVSTIMATSSIQMRFPSYTYN 190826 Query 249 DLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALT----------------------- 285 + L ++VP + H+ + P T + Y A++ Sbjct 190827 SMIGLMSSLVPIRKCHYITCAYTPFTHNDLRDYAAISNVNAASENSTNAVNRSRQSLMKK 191006 Query 286 --VPELTQQMFDAKNMMAACD--PRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYF 341 + ++ +++ K+ + + + P + YL+ ++ +G + K+V + +L ++ KN+ F Sbjct 191007 AGLSDIMRRLLQPKSRVISVENTPLNC-YLSAMSIVQGDVGAKDVHKAILKLKEKNTMQF 191183 Query 342 VEWIPNNVKTAVCDIPP-------RGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAF 394 W P++++ V P +G +++ + N T++ +LFK +Q+ + +R AF Sbjct 191184 APWKPSSMQINVARRSPCLEDGDTQGGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAF 191363 Query 395 LHWYTGEGM--DEM-EFTEAESNMNDLVSEY 422 L Y M D + EF + ++ D+ EY Sbjct 191364 LEQYRKLDMFKDGLEEFDVSRESLLDVTEEY 191456 Score = 50.8 bits (120), Expect(2) = 2e-46, Method: Compositional matrix adjust. Identities = 25/83 (30%), Positives = 42/83 (51%), Gaps = 18/83 (22%) Frame = +2 Query 6 HLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQ------------------LERI 47 H Q G + +G +FW + EHG+ P G SD Q ++R Sbjct 189950 HQQTGMLLSVVGMEFWAQLCAEHGLAPDGLPLTSSDQQQQQQQQQQQQQQSPSIPLMDRK 190129 Query 48 NVYYNEATGGNYVPRAVLVDLEP 70 ++++ +A G+Y+PR++L+DLEP Sbjct 190130 DIFFYQAEDGHYIPRSILIDLEP 190198 Score = 148 bits (374), Expect(3) = 9e-46, Method: Compositional matrix adjust. Identities = 106/399 (27%), Positives = 202/399 (51%), Gaps = 44/399 (11%) Frame = -2 Query 66 VDLEPGTMDSVRSGPFGQIFRPDNFVFGQ--SGAGNNWAKGHYTEGAELVDAVLDVVRKE 123 +++ ++++ P+G + P+N Q SGAGNNWA G Y G L + + D++ +E Sbjct 1101381 INIRMQVINNIMQSPYGLLHNPENVYQSQDGSGAGNNWASG-YQSGGRLSEDLFDILDRE 1101205 Query 124 AESCDCLQGFQlthslgggtgsgmgtllisKIREEFPDRIMNTFSVVPS--PKVSDTVVE 181 A++ + L+GF L HS+ GGTGSG+G+ L+ + + FP +++ T+SV PS VSD VV+ Sbjct 1101204 ADNAESLEGFNLMHSIAGGTGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQ 1101025 Query 182 PYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLR 241 PYN+ LS+ +L EN D +DN AL + L + ++ +N +VS M+ + +R Sbjct 1101024 PYNSVLSLKRLTENADMVTVLDNAALNRLAQDQLHIKNASFAQINRMVSTIMATSSIQMR 1100845 Query 242 FPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQY-------------------- 281 FP + L ++VP + H+ ++P T + Y Sbjct 1100844 FPSYTYNSMIGLMSSLVPIRKCHYITCAYSPFTHNDLRDYAASSNVNAAASENSANAVNR 1100665 Query 282 ------RALTVPELTQQMFDAKNMMAACD--PRHGRYLTVAAVFRGRMSMKEVDEQMLSV 333 + + ++ +++ K+ + + + P + +L+ + +G + K+V + +L + Sbjct 1100664 SRQSLMKKAGLSDIMRRLLQTKSRVISVENTPLNC-FLSAMTLVQGDVGAKDVHKAILKL 1100488 Query 334 QSKNSSYFVEWIPNNVKTAVC-------DIPPRGLKMAATFIGNSTAIQELFKRISEQFT 386 + KN+ F W P++++ V D P+ +++ + N T++ +LFK +Q+ Sbjct 1100487 KEKNTMQFAPWKPSSMQINVARRSPCLEDGDPQSGRVSGLMLANHTSVAQLFKSTIDQYD 1100308 Query 387 AMFRRKAFLHWYTGEGM--DEM-EFTEAESNMNDLVSEY 422 + +R AFL Y M D + EF + ++ ++ EY Sbjct 1100307 RLRKRNAFLEQYRKLDMFKDGLEEFDVSRESLLEVTEEY 1100191 Score = 44.7 bits (104), Expect(3) = 9e-46, Method: Compositional matrix adjust. Identities = 21/63 (33%), Positives = 36/63 (57%), Gaps = 8/63 (13%) Frame = -1 Query 16 IGAKFWEVISDEHGIDPTG--------TYHGDSDLQLERINVYYNEATGGNYVPRAVLVD 67 +G +FW + EHG+ P G S ++R ++++ +A G+Y+PR++LVD Sbjct 1101622 VGMEFWAQLCAEHGLAPDGLPLTSSDQQQQSSSPPLMDRKDIFFYQAEDGHYIPRSILVD 1101443 Query 68 LEP 70 LEP Sbjct 1101442 LEP 1101434 Score = 30.0 bits (66), Expect(3) = 9e-46, Method: Compositional matrix adjust. Identities = 11/14 (79%), Positives = 12/14 (86%), Gaps = 0/14 (0%) Frame = -3 Query 2 REIVHLQAGQCGNQ 15 REI+ LQ GQCGNQ Sbjct 1101728 REIISLQVGQCGNQ 1101687 Score = 28.5 bits (62), Expect = 8.5, Method: Compositional matrix adjust. Identities = 11/14 (79%), Positives = 12/14 (86%), Gaps = 0/14 (0%) Frame = +3 Query 2 REIVHLQAGQCGNQ 15 REI+ LQ GQCGNQ Sbjct 189873 REIISLQVGQCGNQ 189914 > scaffold-693 Length=1268102 Score = 30.8 bits (68), Expect = 1.6, Method: Compositional matrix adjust. Identities = 14/60 (23%), Positives = 30/60 (50%), Gaps = 10/60 (17%) Frame = +1 Query 304 DPRHGRYLTVAAVFRGRMSMKEVDE----------QMLSVQSKNSSYFVEWIPNNVKTAV 353 D + R A++ R R S++ V+ +++Q +S++ + W PNN +T++ Sbjct 510667 DSQRLRSTAAASLLRHRCSLQAVNRIRIRTQWEFWNQINLQRSSSTFSINWAPNNARTSI 510846 Lambda K H a alpha 0.320 0.135 0.407 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2674958559 Database: X5.fasta Posted date: Dec 10, 2019 3:48 AM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40