TBLASTN 2.2.28+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: X5.fasta
           1,868 sequences; 23,962,143 total letters



Query= H2A_ENCCU Q8SSG3 Histone H2A

Length=128
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  scaffold-57                                                         45.4    2e-06
  unplaced-5                                                          36.6    0.002
  scaffold-423                                                        27.3    2.3  
  unplaced-71                                                         26.6    5.1  
  scaffold-633                                                        26.2    5.9  


> scaffold-57
Length=110052

 Score = 45.4 bits (106),  Expect = 2e-06, Method: Composition-based stats.
 Identities = 30/94 (32%), Positives = 48/94 (51%), Gaps = 0/94 (0%)
 Frame = -2

Query  33     KKIMKDRTRMRISKDALVAVSACVMYLISEITDGAKNVASTDGKKKVMPKHINNAICNDT  92
              +K+ +     RI   A V ++A + YL +EI + A N A  + K +++P+H+  AI ND 
Sbjct  91922  RKLRQGNYAQRIGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDE  91743

Query  93     ELHFVGHDWLIKNGGMKSYIAPGDFAVSSKKGSS  126
              EL+ +  D  I  GG+   I        SK  +S
Sbjct  91742  ELNKLLGDVTIAQGGVLPNIHTTLLPKKSKSAAS  91641


 Score = 45.4 bits (106),  Expect = 2e-06, Method: Composition-based stats.
 Identities = 30/94 (32%), Positives = 48/94 (51%), Gaps = 0/94 (0%)
 Frame = +3

Query  33     KKIMKDRTRMRISKDALVAVSACVMYLISEITDGAKNVASTDGKKKVMPKHINNAICNDT  92
              +K+ +     RI   A V ++A + YL +EI + A N A  + K +++P+H+  AI ND 
Sbjct  78939  RKLRQGNYAQRIGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDE  79118

Query  93     ELHFVGHDWLIKNGGMKSYIAPGDFAVSSKKGSS  126
              EL+ +  D  I  GG+   I        SK  +S
Sbjct  79119  ELNKLLGDVTIAQGGVLPNIHTTLLPKKSKSAAS  79220


> unplaced-5
Length=12763

 Score = 36.6 bits (83),  Expect = 0.002, Method: Compositional matrix adjust.
 Identities = 22/66 (33%), Positives = 34/66 (52%), Gaps = 0/66 (0%)
 Frame = +2

Query  61   SEITDGAKNVASTDGKKKVMPKHINNAICNDTELHFVGHDWLIKNGGMKSYIAPGDFAVS  120
            +EI + A N A  + K +++P+H+  AI ND EL+ +  D  I  GG+   I        
Sbjct  2    AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGDVTIAQGGVLPNIHTTLLPKK  181

Query  121  SKKGSS  126
            SK  +S
Sbjct  182  SKSAAS  199


 Score = 36.6 bits (83),  Expect = 0.002, Method: Compositional matrix adjust.
 Identities = 22/66 (33%), Positives = 34/66 (52%), Gaps = 0/66 (0%)
 Frame = -2

Query  61     SEITDGAKNVASTDGKKKVMPKHINNAICNDTELHFVGHDWLIKNGGMKSYIAPGDFAVS  120
              +EI + A N A  + K +++P+H+  AI ND EL+ +  D  I  GG+   I        
Sbjct  12762  AEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGDVTIAQGGVLPNIHTTLLPKK  12583

Query  121    SKKGSS  126
              SK  +S
Sbjct  12582  SKSAAS  12565


> scaffold-423
Length=1446679

 Score = 27.3 bits (59),  Expect = 2.3, Method: Composition-based stats.
 Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (4%)
 Frame = -1

Query  96      FVGHDWLIKNGGMKSYIAPGDFAVSSKK  123
               FV + WLI N  +KSYI+PG   +S  +
Sbjct  842701  FVLYAWLIVNC-LKSYISPGLLGISKSR  842621


> unplaced-71
Length=28457

 Score = 26.6 bits (57),  Expect = 5.1, Method: Composition-based stats.
 Identities = 17/63 (27%), Positives = 29/63 (46%), Gaps = 3/63 (5%)
 Frame = +2

Query  29    LSQIKKIMKDRT---RMRISKDALVAVSACVMYLISEITDGAKNVASTDGKKKVMPKHIN  85
             L++IKKIM +       R+  +A+VA +AC    +             DG+K +  K + 
Sbjct  5558  LARIKKIMNEEAVGNGTRVQGEAIVAAAACTEVWLDYFIQHIYQYTKRDGRKGISYKDVA  5737

Query  86    NAI  88
              A+
Sbjct  5738  LAV  5746


> scaffold-633
Length=196382

 Score = 26.2 bits (56),  Expect = 5.9, Method: Composition-based stats.
 Identities = 19/66 (29%), Positives = 26/66 (39%), Gaps = 0/66 (0%)
 Frame = -1

Query  35     IMKDRTRMRISKDALVAVSACVMYLISEITDGAKNVASTDGKKKVMPKHINNAICNDTEL  94
              I+ DR    I K  L+      +  IS  T   K + +    K V   HI N +C D   
Sbjct  39080  IILDRVEFIIQKTQLIFGQFTSLRHISNCTSLCKRLNTMHDFKLVTLSHIFNTVCGDGRE  38901

Query  95     HFVGHD  100
                 G+D
Sbjct  38900  FPTGND  38883



Lambda      K        H        a         alpha
   0.316    0.132    0.375    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 328722786


  Database: X5.fasta
    Posted date:  Feb 18, 2021  11:04 PM
  Number of letters in database: 23,962,143
  Number of sequences in database:  1,868



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40