Running MakePangenome.. Information about input data: Number of genomes: 3 Number of sequences: 3 Number of sequences per genome: 1 Total length of input (bp): 12767713 Minimum genome length (bp): 4221278 Median genome length (bp): 4263919 Average genome length (bp): 4255904 Maximum genome length (bp): 4282516 ============================ [2021-Dec-14 11:49:17 UTC+03:00] BeginIteration 1, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 440882 Stable blocks (represented in all genomes once, but not minor): fragments: 1140 blocks: 380 Block identity: min: 0.85 median: 0.9185 avg: 0.9154 max: 0.9857 Identity of joined blocks: 0.931831 Nucleotides in blocks: 1254421 The percentage of input length: 9.82% ============================ [2021-Dec-14 11:50:04 UTC+03:00] BeginIteration 2, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 3401057 Stable blocks (represented in all genomes once, but not minor): fragments: 5589 blocks: 1863 Block identity: min: 0.8245 median: 0.922 avg: 0.9191 max: 0.9926 Identity of joined blocks: 0.935449 Nucleotides in blocks: 9664358 The percentage of input length: 75.69% Distance from previous pre-pangenome (bp): 8296607 The percentage of input length: 64.98% Distance is sufficient to carry on ============================ [2021-Dec-14 11:51:25 UTC+03:00] BeginIteration 3, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 3999446 Stable blocks (represented in all genomes once, but not minor): fragments: 6243 blocks: 2081 Block identity: min: 0.8245 median: 0.9215 avg: 0.9186 max: 0.9926 Identity of joined blocks: 0.935085 Nucleotides in blocks: 10644894 The percentage of input length: 83.37% Distance from previous pre-pangenome (bp): 1301121 The percentage of input length: 10.19% Distance is sufficient to carry on ============================ [2021-Dec-14 11:52:26 UTC+03:00] BeginIteration 4, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 4108759 Stable blocks (represented in all genomes once, but not minor): fragments: 6438 blocks: 2146 Block identity: min: 0.8245 median: 0.9214 avg: 0.9185 max: 0.9926 Identity of joined blocks: 0.934929 Nucleotides in blocks: 10863023 The percentage of input length: 85.08% Distance from previous pre-pangenome (bp): 244123 The percentage of input length: 1.91% Distance is sufficient to carry on ============================ [2021-Dec-14 11:53:22 UTC+03:00] BeginIteration 5, AnchorJoiner Length of all major blocks of at least 2 fragments: 4137120 Stable blocks (represented in all genomes once, but not minor): fragments: 6444 blocks: 2148 Block identity: min: 0.8245 median: 0.9214 avg: 0.9185 max: 0.9955 Identity of joined blocks: 0.93492 Nucleotides in blocks: 10861691 The percentage of input length: 85.07% Distance from previous pre-pangenome (bp): 59711 The percentage of input length: 0.47% Distance is sufficient to carry on ============================ [2021-Dec-14 11:56:11 UTC+03:00] BeginIteration 6, AnchorJoiner Length of all major blocks of at least 2 fragments: 4137192 Stable blocks (represented in all genomes once, but not minor): fragments: 6447 blocks: 2149 Block identity: min: 0.8245 median: 0.9214 avg: 0.9185 max: 0.9955 Identity of joined blocks: 0.934921 Nucleotides in blocks: 10861609 The percentage of input length: 85.07% Distance from previous pre-pangenome (bp): 2615 The percentage of input length: 0.02% Distance is too low => stopping this loop ============================ [2021-Dec-14 11:57:06 UTC+03:00] BeginIteration 7, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 4136526 Stable blocks (represented in all genomes once, but not minor): fragments: 6489 blocks: 2163 Block identity: min: 0.807 median: 0.9214 avg: 0.9182 max: 0.9955 Identity of joined blocks: 0.93488 Nucleotides in blocks: 10864738 The percentage of input length: 85.09% Distance from previous pre-pangenome (bp): 58960 The percentage of input length: 0.46% Distance is sufficient to carry on ============================ [2021-Dec-14 11:59:10 UTC+03:00] BeginIteration 8, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 4136602 Stable blocks (represented in all genomes once, but not minor): fragments: 6489 blocks: 2163 Block identity: min: 0.807 median: 0.9214 avg: 0.9181 max: 0.9955 Identity of joined blocks: 0.934871 Nucleotides in blocks: 10865192 The percentage of input length: 85.09% Distance from previous pre-pangenome (bp): 724 The percentage of input length: 0.01% Distance is too low => stopping this loop ============================ [2021-Dec-14 12:02:30 UTC+03:00] StopInfo The pangenome has been constructed. Blocks' lengths: 4747399 major: 4738595 minor: 8804 ============================ All major blocks of at least 2 fragments: fragments: 2840 blocks: 998 fragments / blocks: 2.8456 Block identity: min: 0.8058 median: 0.9393 avg: 0.9484 max: 1 Identity of joined blocks: 0.939832 Block sizes: min: 2 median: 3 avg: 2.85 max: 30 Fragment lengths: min: 76 median: 608 avg: 4289.06 max: 198866 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0051 max: 0.3333 GC content: min: 0.2255 median: 0.4166 avg: 0.4115 max: 0.7321 Nucleotides in blocks: 12180928 The percentage of input length: 95.4% Total length of blocks: 4167455 The percentage of total blocks' length: 87.78% ============================ Stable blocks (represented in all genomes once, but not minor): fragments: 1449 blocks: 483 fragments / blocks: 3 Block identity: min: 0.807 median: 0.9238 avg: 0.9178 max: 0.9955 Identity of joined blocks: 0.932278 Block sizes: 3 Fragment lengths: min: 80 median: 2206 avg: 7557.01 max: 198866 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0.0007 avg: 0.0062 max: 0.3333 GC content: min: 0.2322 median: 0.4334 avg: 0.4217 max: 0.5631 Nucleotides in blocks: 10950103 The percentage of input length: 85.76% Total length of blocks: 3657231 The percentage of total blocks' length: 77.03% ============================ Partial blocks (represented once in subset of genomes): fragments: 804 blocks: 402 fragments / blocks: 2 Block identity: min: 0.8381 median: 1 avg: 0.9985 max: 1 Identity of joined blocks: 0.99928 Block sizes: 2 Fragment lengths: min: 100 median: 255 avg: 1193.31 max: 40867 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0003 max: 0.072 GC content: min: 0.2255 median: 0.3816 avg: 0.3896 max: 0.7321 Nucleotides in blocks: 959419 The percentage of input length: 7.51% Total length of blocks: 479793 The percentage of total blocks' length: 10.1% ============================ Blocks with repeats (at least two copies in at least one genome): fragments: 587 blocks: 113 fragments / blocks: 5.1946 Block identity: min: 0.8058 median: 0.8913 avg: 0.9013 max: 1 Identity of joined blocks: 0.910343 Block sizes: min: 2 median: 4 avg: 5.19 max: 30 Fragment lengths: min: 76 median: 157 avg: 462.36 max: 3250 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0173 max: 0.3176 GC content: min: 0.2606 median: 0.4306 avg: 0.4453 max: 0.6411 Nucleotides in blocks: 271406 The percentage of input length: 2.12% Total length of blocks: 30431 The percentage of total blocks' length: 0.64% ============================ Unique (blocks of 1 fragment but not minor): fragments: 417 blocks: 417 Fragment lengths: min: 100 median: 312 avg: 1369.64 max: 42985 GC content: min: 0.1968 median: 0.387 avg: 0.392 max: 0.7132 Nucleotides in blocks: 571140 The percentage of input length: 4.47% Total length of blocks: 571140 The percentage of total blocks' length: 12.03% ============================ Minor blocks (too short to say smth about): fragments: 481 blocks: 233 fragments / blocks: 2.0643 Block identity: min: 0 median: 1 avg: 0.8831 max: 1 Identity of joined blocks: 0.954674 Block sizes: min: 1 median: 2 avg: 2.06 max: 26 Fragment lengths: min: 1 median: 22 avg: 32.53 max: 99 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0404 max: 1.25 GC content: min: 0 median: 0.4166 avg: 0.4228 max: 1 Nucleotides in blocks: 15645 The percentage of input length: 0.12% Total length of blocks: 8804 The percentage of total blocks' length: 0.18% The pangenome has been prepared for the next step: PostProcessing .. done