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Archaeon S. acidocaldarius
Source: Biological diversity: bacteria and archaeans
Scientific classification [UniProt]
> cellular organisms
> Archaea
> Crenarchaeota
> Thermoprotei
> Sulfolobales
> Sulfolobaceae
> Sulfolobus
> Sulfolobus acidocaldarius
General information [UniProt]
Scientific name: Sulfolobus acidocaldarius.
Common name: N/A.
Characteristics [EBI]
Sulfolobus acidocaldarius is a model for research on mechanisms of DNA replication.
Sulfolobus acidocaldarius is an aerobic thermoacidophilic crenarchaeon. Strain DSM639, was the first hyperthermoacidophile
to be characterised from terrestrial solfataras.
It grows optimally at 75 to 80°C and pH 2 to 3, under strictly aerobic conditions, on complex organic substrates, including
yeast extract, tryptone, and Casamino Acids and a limited number of sugars.
Many of the seminal studies on archaea and crenarchaea were performed on S. acidocaldarius. It is used to demonstrate the
similarity of the archaeal and eukaryal transcription apparatuses. Also, its sensitivity to a wide range of ribosomal
antibiotics and ease of transformation have made S. acidocaldarius a focus for in vivo genetic studies.
S. acidocaldarius has also been used for studying genetic fidelity at high temperatures and is the only hyperthermophilic
archaeon for which the rate and type of spontaneous mutation have been quantified in vivo. Its relatively low mutation
rate, despite its high-temperature environment, has stimulated a strong interest in its efficient repair systems. Special
features include its ability to exchange chromosomal genes intercellularly and its capacity to grow synchronously in
culture which has facilitated archaeal cell cycle studies.
Determination of the genome sequence of S. acidocaldarius and comparative studies with the genomes of S. solfataricus
and S. tokodaii, have made it possible to generate a public database for the Sulfolobus genomes which will serve as a
important research resource.
PubMed resource
The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota is one of the PudMed articles about S. acidocaldarius
written by Chen L, Brugger K, Skovgaard M, Redder P, She Q, Torarinsson E, Greve B, Awayez M, Zibat A, Klenk HP, Garrett RA.
Identifier: 20936123
Abstract: Glycosylation of the S-layer of the crenarchaea Sulfolobus acidocaldarius has been investigated using glycoproteomic methodologies. The mature protein is predicted to contain 31 N-glycosylation consensus sites with approximately one third being found in the C-terminal domain spanning residues L(1004)-Q(1395). Since this domain is rich in Lys and Arg and therefore relatively tractable to glycoproteomic analysis, this study has focused on mapping its N-glycosylation. Our analysis identified nine of the 11 consensus sequence sites, and all were found to be glycosylated. This constitutes a remarkably high glycosylation density in the C-terminal domain averaging one site for each stretch of 30-40 residues. Each of the glycosylation sites observed was shown to be modified with a heterogeneous family of glycans, with the largest having a composition Glc(1)Man(2)GlcNAc(2) plus 6-sulfoquinovose (QuiS), consistent with the tribranched hexasaccharide previously reported in the cytochrome b(558/566) of S. acidocaldarius. S. acidocaldarius is the only archaeal species whose N-glycans are known to be linked via the chitobiose core disaccharide that characterises the N-linked glycans of Eukarya.
Google search query: site:http://www.ncbi.nlm.nih.gov/pubmed "Sulfolobus acidocaldarius"
New information (block 2)
© Anton Vasetenkov
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