|   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   |   | 2 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   |   | 4 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   |   | 6 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   |   | 8 | 0 |   |   |   |   |   |   |   |
P | R | O | T | _ | D | A | L | I |   |   | : |   | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | A | P | F | F | D | L | G | E | L | K | L | W | S | F | W | R | A | A | I | A | E | F | I | A | T | L | L | F | L | Y | I | T | V | A | T | V | I | G | H | S | K | E | T | V | V | C | G | S | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 6 | 1 |
P | I | P | 2 | 8 | _ | A | R | A | T |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
p | e | p | w | i | n | d | o | w |   |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
h | a | n | d | s |   |   |   |   |   |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
T | M | H | M | M |   |   |   |   |   |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
O | P | M |   |   |   |   |   |   |   |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
  |   |   |   |   |   |   |   |   |   |   | : |   | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |   | : |   |   |   | - |
f | i | n | a | l |   |   |   |   |   |   | : |   | M | S | K | E | V | S | E | E | G | R | H | G | K | D | Y | V | D | P | P | P | A | P | L | L | D | M | A | E | L | K | L | W | S | F | Y | R | A | I | I | A | E | F | I | A | T | L | L | F | L | Y | V | T | V | A | T | V | I | G | H | K | N | Q | T | G | P | C | G | G | V | G | L | L | G | I | A | W | A | F | G | G | M |   | : |   |   | 8 | 1 |
  |   |   |   |   |   |   |   |   |   |   |   |   | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0 | 0 | 0 | 0 | 0 | 0 | 0 |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 1 | 0 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 1 | 2 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 1 | 4 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 1 | 6 | 0 |   |   |   |   |   |   |   |   |
P | R | O | T | _ | D | A | L | I |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | L | R | A | L | V | Y | M | I | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | K | G | P | Y | N | Q | F | G | G | G | A | N | S | V | A | L | G | Y | N | K | G | T | A | L |   | : |   | 1 | 4 | 2 |
P | I | P | 2 | 8 | _ | A | R | A | T |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
p | e | p | w | i | n | d | o | w |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
h | a | n | d | s |   |   |   |   |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
T | M | H | M | M |   |   |   |   |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
O | P | M |   |   |   |   |   |   |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
  |   |   |   |   |   |   |   |   |   |   | : |   | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |   | : |   |   |   | - |
f | i | n | a | l |   |   |   |   |   |   | : |   | I | F | V | L | V | Y | C | T | A | G | I | S | G | G | H | I | N | P | A | V | T | F | G | L | F | L | A | R | K | V | S | L | P | R | A | V | A | Y | M | V | A | Q | C | L | G | A | I | C | G | V | G | L | V | K | A | F | M | M | T | P | Y | K | R | L | G | G | G | A | N | T | V | A | D | G | Y | S | T | G | T | A | L |   | : |   | 1 | 6 | 2 |
  |   |   |   |   |   |   |   |   |   |   |   |   | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0 | 0 | 0 | 0 |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 1 | 8 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 2 | 0 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 2 | 2 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 2 | 4 | 0 |   |   |   |   |   |   |   |   |   |
P | R | O | T | _ | D | A | L | I |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | I | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | F | N | S | N | K | V | W | D | D | Q | W | I | F | W |   | : |   | 2 | 2 | 3 |
P | I | P | 2 | 8 | _ | A | R | A | T |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
p | e | p | w | i | n | d | o | w |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
h | a | n | d | s |   |   |   |   |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
T | M | H | M | M |   |   |   |   |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
O | P | M |   |   |   |   |   |   |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
  |   |   |   |   |   |   |   |   |   |   | : |   | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |   | : |   |   |   | - |
f | i | n | a | l |   |   |   |   |   |   | : |   | G | A | E | I | I | G | T | F | V | L | V | Y | T | V | F | S | A | T | D | P | K | R | S | A | R | D | S | H | V | P | V | L | A | P | L | P | I | G | F | A | V | F | M | V | H | L | A | T | I | P | I | T | G | T | G | I | N | P | A | R | S | F | G | A | A | V | I | Y | N | N | E | K | A | W | D | D | H | W | I | F | W |   | : |   | 2 | 4 | 3 |
  |   |   |   |   |   |   |   |   |   |   |   |   | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | + | + | + | + | + | + | + | + | + | + | + | + | + | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | 0 | 0 | 0 |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |
  |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   | 2 | 6 | 0 |   |   |   |   |   |   |   |   |   | * |   |   |   |   |   |   |   |   |   |   |   |   |   |   |
P | R | O | T | _ | D | A | L | I |   |   | : |   | V | G | P | F | I | G | A | A | V | A | A | A | Y | H | Q | Y | V | L | R | A | A | A | I | K | A | L | G | S | - | - | - | - | - | - | - |   | : |   | 2 | 5 | 1 |
P | I | P | 2 | 8 | _ | A | R | A | T |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
p | e | p | w | i | n | d | o | w |   |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
h | a | n | d | s |   |   |   |   |   |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
T | M | H | M | M |   |   |   |   |   |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
O | P | M |   |   |   |   |   |   |   |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
  |   |   |   |   |   |   |   |   |   |   | : |   | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . |   | : |   |   |   | - |
f | i | n | a | l |   |   |   |   |   |   | : |   | V | G | P | F | V | G | A | L | A | A | A | A | Y | H | Q | Y | I | L | R | A | A | A | I | K | A | L | A | S | F | R | S | N | P | T | N |   | : |   | 2 | 7 | 8 |
  |   |   |   |   |   |   |   |   |   |   |   |   | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |   |   |   |   |   |   |
Первая строка последовательность белка-прототипа.
Вторая белка PIP28_ARATH.
Серым |
Третья cтрока предсказанная почти вручную топология на основе данных программы pepwindow.
Красным |
Розовым |
Голубым |
Желтым |
На четвертой строке дана окончательная Обработка вручную.
Зеленым |
Пятая строка размечена в соответствии с результатами TMHMM.
Шестая строка в соответствии с OPM.
На предпоследней строке показан окончательный вариант местонахождения трансмембранных участков на основании всех методов и с учетом профиля гидрофобности, свойств аминокислотных остатков и толщины мембраны (она составляет примерно 20 а.о.).
На последней строке указаны цитоплазматические участки (+), трансмембранные участки (0) и внеклеточные фрагменты (-). Данные получены с помощью ExPASy.
Эти данные были получены сразу после данных pepwindow. Последующих данных на тот момент не было.
Как видно по рисунку, пропущено 2 трансмембранных участка: 159-179 и 241-261.
Cогласно с профилем гидрофобности и с гидрофобностью/гидрофильностью аминокислотных остатков эти фрагменты были добавлены с несколько измененными границами.