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Alignment of homologous proteins sequences
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- A 6-sequence alignment was built with proteins chosen from HSP70 family from Eukaryota, Bacteria and Archea. The coloured alignment by ClustalX scheme with Identity Threshold = 100% condition is presented on picture. The colour scheme is such that only coloumns with 100% identical aminoacid residues in each sequence are coloured.
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- A markup Labels was added. Letter C marks positions, which are more than 80% conservative, F is for functionally conservative ones and G means gaps. There are valine, leucine and isoleucine (hydrophobic amino acids) on positions 69,77 and 78.
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- Main properties of this alignment are presented in the following table:
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| ID |
100% conservative |
% |
Gaps |
% |
Functionally conservative |
% |
70% conservative |
% |
| B2VGR9 |
178 |
23,7 |
114 |
15,18 |
290 |
38,6 |
344 |
45,8 |
| A67226 |
178 |
23,7 |
101 |
13,45 |
290 |
38,6 |
345 |
45,9 |
| Q3IUI0 |
178 |
23,7 |
95 |
12,65 |
290 |
38,6 |
310 |
41,3 |
| P11021 |
178 |
23,7 |
97 |
12,92 |
290 |
38,6 |
305 |
40,6 |
| P38646 |
178 |
23,7 |
72 |
9,59 |
290 |
38,6 |
340 |
45,3 |
| Q6L0S7 |
178 |
23,7 |
138 |
18,38 |
290 |
38,6 |
302 |
40,2 |
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Evolution of the protein sequence
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- Automaticly built alignment:
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- Manually built alignment:
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Fulfilled corrections:
- On positions 20, 21, 22 serine and valine were replaced to the left, because deletion and insertion are more feasible than mutation.
- On positions 120 and 121 the same thing was done with leucine.
- On positions 228 229 leucine and glutamic acid were also replaced to the left.
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Mutations order:
- On position 195 occured phenylalanine insertion (between original sequence and ancestor 1)
- On position 6 occured alanine insertion (between ancestor 4 and 5)
- On position 45 occured mutation from arginine to cysteine (between ancestor 5 and 6)
- On position 159 occured delecion of aspartic acid (between ancestor 1 and 2)
- On position 20 occured serine insertion (between ancestor 3 and 4)
- On position 31 occured lesine insertion (between ancestor 4 and 5)
- On position 88 occured alanine insertion (between ancestor 6 and 7)
- On position 207 occured threonine deletion (between ancestor 4 and 5)
- On position 209 occured mutation from glycine to asparagine (between ancestor 6 and 7)
- On position 130 occured isoleucine insertion (between ancestor 2 and 3)
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