Plural alignment's algorithms. Pfam.

		

Plural alignments build by a different programs.

		

		
I have chosen 5 different proteins from work 10 and built two different alignments. First was made by Tcofee program, the second one - by GLprobs.
		

		
  • Tcoffee regards first methionines as homologous in contradistinction to Glprobs, which does not separate them from all other sequence, thus it diminishes the number of inner gaps.
  • On position 326-327 we can observe a different alignment in case of glicines, where Glprobs places them all together, while Tcoffee spreads those on not neighboring positions.
  • On positions 245-250 Glprobs alignes glutamines in a different way than Tcoffee does, which places them all together on positions 247-248.
		

		
Alignment link
		

		

Domain architecture

		

		
For research I have investigated Xanthomonas citri pv. citri carboxylesterase protein's architecture (A0A1L6GII5). It has one domain Abhydrolase_2 (PF02230), on positions 2-219
		

		
There are 4683 sequences with the following architecture: Abhydrolase_2
		
		
There are 157 sequences with the following architecture: Abhydrolase_2 x 2
		
		
There are 19 sequences with the following architecture: Abhydrolase_2, Peptidase_S9
		


© Popov Nikita 2016