Sanger sequencing.

		
Both chromatogrammes reveal a pretty high quality, which nevetheless becomes smudged on both ends. There was just one interresting place (before and after variants are presented below) on positions 663 - 670 (false chromatograme) insertion of two excess cytosines was observed and fixed, according to the right one. Noise level is low, complete lack of mistakes connected with paint spots observed. The lenth of non-reading ends is around 10-15 bases. Noise level is approximatly 10-13 times less than signal.
		

		
Except this case there were some places with polymorphisms and nonrecognised nucleotides, but all of them are quite typical. Fixed and previous versions of chromatogrammes can be found in attached files. As an exapmle this case can also be considered: on position 326 (false one) polymorphysm was observed, as well as on position 348, but both of them can be uniquely rebuilt with the help of the second chromatogramm
		

		
There are also screenshots of completed ends of both chromatogrames:
		

WSWS2960_COI_F_G11_WSBS-Seq-1-08-15.ab1 Download
WSWS2960_COI_R_G12_WSBS-Seq-1-08-15.ab1 Download
ws2960.fasta Download
WSF.fasta Download
WSR.fasta Download
ws.mfa Download


© Popov Nikita 2016